منابع مشابه
Bacterial flavodoxins support nitric oxide production by Bacillus subtilis nitric-oxide synthase.
Unlike animal nitric-oxide synthases (NOSs), the bacterial NOS enzymes have no attached flavoprotein domain to reduce their heme and so must rely on unknown bacterial proteins for electrons. We tested the ability of two Bacillus subtilis flavodoxins (YkuN and YkuP) to support catalysis by purified B. subtilis NOS (bsNOS). When an NADPH-utilizing bacterial flavodoxin reductase (FLDR) was added t...
متن کاملInduction of Nitric Oxide Synthase by Bacterial Lipopolysaccharide
This study investigates the role of endogenous platelet-activating factor (PAF) in the production of nitric oxide (NO) by constitutive and inducible isoforms of NO synthase (NOS) in endotoxin shock. In anesthetized rats, 3 hours of endotoxemia resulted in a fall in mean arterial blood pressure (MAP) from 127±5 (control) to 61±7 mm Hg and a reduction of the pressor responses to norepinephrine (N...
متن کاملBacterial infection induces nitric oxide synthase in human neutrophils.
The identification of human inflammatory cells that express inducible nitric oxide synthase and the clarification of the role of inducible nitric oxide synthase in human infectious or inflammatory processes have been elusive. In neutrophil-enriched fractions from urine, we demonstrate a 43-fold increase in nitric oxide synthase activity in patients with urinary tract infections compared with th...
متن کاملStructure-Based Design of Bacterial Nitric Oxide Synthase Inhibitors
Inhibition of bacterial nitric oxide synthase (bNOS) has the potential to improve the efficacy of antimicrobials used to treat infections by Gram-positive pathogens Staphylococcus aureus and Bacillus anthracis. However, inhibitor specificity toward bNOS over the mammalian NOS (mNOS) isoforms remains a challenge because of the near identical NOS active sites. One key structural difference betwee...
متن کاملStructural and biological studies on bacterial nitric oxide synthase inhibitors.
Nitric oxide (NO) produced by bacterial NOS functions as a cytoprotective agent against oxidative stress in Staphylococcus aureus, Bacillus anthracis, and Bacillus subtilis. The screening of several NOS-selective inhibitors uncovered two inhibitors with potential antimicrobial properties. These two compounds impede the growth of B. subtilis under oxidative stress, and crystal structures show th...
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ژورنال
عنوان ژورنال: Microbiology&Biotechnology
سال: 2011
ISSN: 2307-4663,2076-0558
DOI: 10.18524/2307-4663.2011.3(15).92878